| Train | Source | Destination | Days Run from Pune Jn |
| 22139 - Ajni Humsafar | Pune Jn (PUNE) | Ajni (AJNI) | Sat |
| # | Station | Arrival | Departure | Halt | Distance | Day |
| 1 | Pune Jn (PUNE) | Source | 10:00 PM | 0 Mins | 0 KMs | 1 |
| 2 | Daund Chord Line (DDCC) | 11:03 PM | 11:05 PM | 2 Mins | 72 KMs | 1 |
| 3 | Manmad Jn (MMR) | 03:30 AM | 03:35 AM | 5 Mins | 307 KMs | 2 |
| 4 | Chalisgaon Jn (CSN) | 04:24 AM | 04:25 AM | 1 Mins | 374 KMs | 2 |
| 5 | Bhusaval Jn (BSL) | 06:05 AM | 06:10 AM | 5 Mins | 491 KMs | 2 |
| 6 | Akola Jn. (AK) | 08:33 AM | 08:35 AM | 2 Mins | 631 KMs | 2 |
| 7 | Badnera Jn. (BD) | 09:58 AM | 10:00 AM | 2 Mins | 710 KMs | 2 |
| 8 | Wardha Jn (WR) | 11:13 AM | 11:15 AM | 2 Mins | 805 KMs | 2 |
| 9 | Ajni (AJNI) | 12:50 PM | Destination | 0 Mins | 881 KMs | 2 |