| Train | Source | Destination | Days Run from Pune Jn |
| 22123 - Pune Ajni Ac Express | Pune Jn (PUNE) | Ajni (AJNI) | Fri |
| # | Station | Arrival | Departure | Halt | Distance | Day |
| 1 | Pune Jn (PUNE) | Source | 03:15 PM | 0 Mins | 0 KMs | 1 |
| 2 | Daund Chord Line (DDCC) | 04:13 PM | 04:15 PM | 2 Mins | 72 KMs | 1 |
| 3 | Kopargaon (KPG) | 06:57 PM | 07:00 PM | 3 Mins | 265 KMs | 1 |
| 4 | Manmad Jn (MMR) | 08:15 PM | 08:20 PM | 5 Mins | 307 KMs | 1 |
| 5 | Bhusaval Jn (BSL) | 10:35 PM | 10:40 PM | 5 Mins | 491 KMs | 1 |
| 6 | Nandura (NN) | 11:44 PM | 11:45 PM | 1 Mins | 569 KMs | 1 |
| 7 | Akola Jn. (AK) | 12:47 AM | 12:50 AM | 3 Mins | 631 KMs | 2 |
| 8 | Badnera Jn. (BD) | 02:00 AM | 02:02 AM | 2 Mins | 710 KMs | 2 |
| 9 | Dhamangaon (DMN) | 02:41 AM | 02:42 AM | 1 Mins | 755 KMs | 2 |
| 10 | Wardha Jn (WR) | 03:19 AM | 03:21 AM | 2 Mins | 805 KMs | 2 |
| 11 | Ajni (AJNI) | 04:50 AM | Destination | 0 Mins | 881 KMs | 2 |