| Train | Source | Destination | Days Run from Madurai Jn |
| 16729 - Mdu Puu Express | Madurai Jn (MDU) | Punalur (PUU) | Mon Tue Wed Fri Sat Sun |
| # | Station | Arrival | Departure | Halt | Distance | Day |
| 1 | Madurai Jn (MDU) | Source | 11:15 PM | 0 Mins | 0 KMs | 1 |
| 2 | Tiruparankundram (TDN) | 11:26 PM | 11:27 PM | 1 Mins | 7 KMs | 1 |
| 3 | Tirumangalam (TMQ) | 11:36 PM | 11:37 PM | 1 Mins | 17 KMs | 1 |
| 4 | Virudunagar Jn (VPT) | 11:55 PM | 11:57 PM | 2 Mins | 43 KMs | 1 |
| 5 | Satur (SRT) | 12:13 AM | 12:15 AM | 2 Mins | 70 KMs | 2 |
| 6 | Kovilpatti (CVP) | 12:33 AM | 12:35 AM | 2 Mins | 91 KMs | 2 |
| 7 | Kadambur (KDU) | 12:49 AM | 12:50 AM | 1 Mins | 114 KMs | 2 |
| 8 | Vanchi Maniyachchi Jn (MEJ) | 01:09 AM | 01:10 AM | 1 Mins | 128 KMs | 2 |
| 9 | Tirunelveli Jn (TEN) | 01:40 AM | 01:45 AM | 5 Mins | 157 KMs | 2 |
| 10 | Nanguneri (NNN) | 02:09 AM | 02:10 AM | 1 Mins | 185 KMs | 2 |
| 11 | Valliyur (VLY) | 02:23 AM | 02:25 AM | 2 Mins | 199 KMs | 2 |
| 12 | Aralvaymoli (AAY) | 02:39 AM | 02:40 AM | 1 Mins | 217 KMs | 2 |
| 13 | Nager Coil Town (NJT) | 04:31 AM | 04:36 AM | 5 Mins | 235 KMs | 2 |
| 14 | Eraniel (ERL) | 04:51 AM | 04:52 AM | 1 Mins | 248 KMs | 2 |
| 15 | Kulitthurai (KZT) | 05:06 AM | 05:07 AM | 1 Mins | 263 KMs | 2 |
| 16 | Kulitturai West (KZTW) | 05:10 AM | 05:11 AM | 1 Mins | 266 KMs | 2 |
| 17 | Parassala (PASA) | 05:15 AM | 05:16 AM | 1 Mins | 271 KMs | 2 |
| 18 | Neyyattinkara (NYY) | 05:34 AM | 05:35 AM | 1 Mins | 284 KMs | 2 |
| 19 | Thiruvananthapuram Central (TVC) | 06:20 AM | 06:25 AM | 5 Mins | 301 KMs | 2 |
| 20 | Thiruvananthapuram Pettah (TVP) | 06:30 AM | 06:31 AM | 1 Mins | 303 KMs | 2 |
| 21 | Thiruvananthapuram North (TVCN) | 06:39 AM | 06:40 AM | 1 Mins | 307 KMs | 2 |
| 22 | Kazhakuttam (KZK) | 06:47 AM | 06:48 AM | 1 Mins | 314 KMs | 2 |
| 23 | Kaniyapuram (KXP) | 06:51 AM | 06:52 AM | 1 Mins | 318 KMs | 2 |
| 24 | Murukkampuzha (MQU) | 07:04 AM | 07:05 AM | 1 Mins | 322 KMs | 2 |
| 25 | Chirayinkeezh (CRY) | 07:11 AM | 07:12 AM | 1 Mins | 330 KMs | 2 |
| 26 | Kadakavur (KVU) | 07:24 AM | 07:25 AM | 1 Mins | 333 KMs | 2 |
| 27 | Varkala (VAK) | 07:39 AM | 07:40 AM | 1 Mins | 342 KMs | 2 |
| 28 | Edavai (EVA) | 07:44 AM | 07:45 AM | 1 Mins | 346 KMs | 2 |
| 29 | Paravur (PVU) | 07:59 AM | 08:00 AM | 1 Mins | 353 KMs | 2 |
| 30 | Mayyanad (MYY) | 08:04 AM | 08:05 AM | 1 Mins | 357 KMs | 2 |
| 31 | Iravipuram (IRP) | 08:10 AM | 08:11 AM | 1 Mins | 361 KMs | 2 |
| 32 | Kollam Jn (QLN) | 08:30 AM | 08:35 AM | 5 Mins | 365 KMs | 2 |
| 33 | Kilikollur (KLQ) | 08:36 AM | 08:37 AM | 1 Mins | 371 KMs | 2 |
| 34 | Kundara (KUV) | 08:45 AM | 08:46 AM | 1 Mins | 378 KMs | 2 |
| 35 | Ezhukone (EKN) | 08:54 AM | 08:55 AM | 1 Mins | 383 KMs | 2 |
| 36 | Kottarakara (KKZ) | 09:02 AM | 09:03 AM | 1 Mins | 390 KMs | 2 |
| 37 | Kuri (KIF) | 09:08 AM | 09:09 AM | 1 Mins | 397 KMs | 2 |
| 38 | Auvaneswsarem (AVS) | 09:19 AM | 09:20 AM | 1 Mins | 401 KMs | 2 |
| 39 | Punalur (PUU) | 09:55 AM | Destination | 0 Mins | 409 KMs | 2 |