| Train | Source | Destination | Days Run from Madurai Jn |
| 16327 - Mdu Guv Express | Madurai Jn (MDU) | Guruvayur (GUV) | Mon Tue Wed Fri Sat Sun |
| # | Station | Arrival | Departure | Halt | Distance | Day |
| 1 | Madurai Jn (MDU) | Source | 11:35 AM | 0 Mins | 0 KMs | 1 |
| 2 | Tiruparankundram (TDN) | 11:44 AM | 11:45 AM | 1 Mins | 7 KMs | 1 |
| 3 | Tirumangalam (TMQ) | 11:54 AM | 11:55 AM | 1 Mins | 17 KMs | 1 |
| 4 | Kalligudi (KGD) | 12:03 PM | 12:04 PM | 1 Mins | 32 KMs | 1 |
| 5 | Virudunagar Jn (VPT) | 12:13 PM | 12:15 PM | 2 Mins | 43 KMs | 1 |
| 6 | Tiruttangal (TTL) | 12:29 PM | 12:30 PM | 1 Mins | 65 KMs | 1 |
| 7 | Sivakasi (SVKS) | 12:39 PM | 12:40 PM | 1 Mins | 67 KMs | 1 |
| 8 | Srivilliputtur (SVPR) | 12:54 PM | 12:55 PM | 1 Mins | 84 KMs | 1 |
| 9 | Rajapalayam (RJPM) | 01:08 PM | 01:10 PM | 2 Mins | 96 KMs | 1 |
| 10 | Sankarankovil (SNKL) | 01:34 PM | 01:35 PM | 1 Mins | 128 KMs | 1 |
| 11 | Pambakovil Shandy (PBKS) | 01:47 PM | 01:48 PM | 1 Mins | 140 KMs | 1 |
| 12 | Kadayanallur (KDNL) | 01:59 PM | 02:00 PM | 1 Mins | 149 KMs | 1 |
| 13 | Tenkasi Jn (TSI) | 02:13 PM | 02:15 PM | 2 Mins | 165 KMs | 1 |
| 14 | Sengottai (SCT) | 02:45 PM | 02:50 PM | 5 Mins | 173 KMs | 1 |
| 15 | Bhagavathipuram (BJM) | 03:09 PM | 03:10 PM | 1 Mins | 179 KMs | 1 |
| 16 | Aryankavu (AYV) | 03:29 PM | 03:30 PM | 1 Mins | 188 KMs | 1 |
| 17 | Aryankavu New Block (AYVN) | 03:41 PM | 03:42 PM | 1 Mins | 192 KMs | 1 |
| 18 | Edapalayam (EDP) | 03:49 PM | 03:50 PM | 1 Mins | 194 KMs | 1 |
| 19 | Kalthuruthy Halt (KTHY) | 03:57 PM | 03:58 PM | 1 Mins | 197 KMs | 1 |
| 20 | Tenmalai (TML) | 04:09 PM | 04:10 PM | 1 Mins | 201 KMs | 1 |
| 21 | Ottakkal (OKL) | 04:20 PM | 04:21 PM | 1 Mins | 206 KMs | 1 |
| 22 | Edamann (EDN) | 04:34 PM | 04:35 PM | 1 Mins | 214 KMs | 1 |
| 23 | Punalur (PUU) | 05:55 PM | 06:00 PM | 5 Mins | 223 KMs | 1 |
| 24 | Auvaneswsarem (AVS) | 06:10 PM | 06:11 PM | 1 Mins | 231 KMs | 1 |
| 25 | Kuri (KIF) | 06:17 PM | 06:18 PM | 1 Mins | 234 KMs | 1 |
| 26 | Kottarakara (KKZ) | 06:27 PM | 06:28 PM | 1 Mins | 242 KMs | 1 |
| 27 | Ezhukone (EKN) | 06:34 PM | 06:35 PM | 1 Mins | 249 KMs | 1 |
| 28 | Kundara East (KFV) | 06:40 PM | 06:41 PM | 1 Mins | 252 KMs | 1 |
| 29 | Kundara (KUV) | 06:49 PM | 06:50 PM | 1 Mins | 254 KMs | 1 |
| 30 | Chandanattop Halt (CTPE) | 06:57 PM | 06:58 PM | 1 Mins | 259 KMs | 1 |
| 31 | Kilikollur (KLQ) | 07:05 PM | 07:06 PM | 1 Mins | 261 KMs | 1 |
| 32 | Kollam Jn (QLN) | 07:35 PM | 07:45 PM | 10 Mins | 267 KMs | 1 |
| 33 | Perinad (PRND) | 07:54 PM | 07:55 PM | 1 Mins | 276 KMs | 1 |
| 34 | Sasthankotta (STKT) | 08:04 PM | 08:05 PM | 1 Mins | 286 KMs | 1 |
| 35 | Karunagappalli (KPY) | 08:16 PM | 08:17 PM | 1 Mins | 294 KMs | 1 |
| 36 | Kayankulam Jn (KYJ) | 08:33 PM | 08:35 PM | 2 Mins | 308 KMs | 1 |
| 37 | Mavelikara (MVLK) | 08:44 PM | 08:45 PM | 1 Mins | 315 KMs | 1 |
| 38 | Cheriyanad (CYN) | 08:49 PM | 08:50 PM | 1 Mins | 322 KMs | 1 |
| 39 | Chengannur (CNGR) | 08:55 PM | 08:57 PM | 2 Mins | 328 KMs | 1 |
| 40 | Tiruvalla (TRVL) | 09:06 PM | 09:07 PM | 1 Mins | 337 KMs | 1 |
| 41 | Changanasseri (CGY) | 09:15 PM | 09:16 PM | 1 Mins | 345 KMs | 1 |
| 42 | Kottayam (KTYM) | 09:42 PM | 09:45 PM | 3 Mins | 362 KMs | 1 |
| 43 | Ettumanur (ETM) | 09:56 PM | 09:57 PM | 1 Mins | 373 KMs | 1 |
| 44 | Kuruppantara (KRPP) | 10:05 PM | 10:06 PM | 1 Mins | 381 KMs | 1 |
| 45 | Vaikam Road (VARD) | 10:14 PM | 10:15 PM | 1 Mins | 387 KMs | 1 |
| 46 | Piravam Road (PVRD) | 10:22 PM | 10:23 PM | 1 Mins | 394 KMs | 1 |
| 47 | Mulanturutti (MNTT) | 10:35 PM | 10:36 PM | 1 Mins | 405 KMs | 1 |
| 48 | Tripunittura (TRTR) | 10:49 PM | 10:50 PM | 1 Mins | 412 KMs | 1 |
| 49 | Ernakulam Town (ERN) | 11:22 PM | 11:25 PM | 3 Mins | 422 KMs | 1 |
| 50 | Idapalli (IPL) | 11:35 PM | 11:36 PM | 1 Mins | 428 KMs | 1 |
| 51 | Kalamasseri (KLMR) | 11:44 PM | 11:45 PM | 1 Mins | 433 KMs | 1 |
| 52 | Aluva (AWY) | 12:05 AM | 12:06 AM | 1 Mins | 439 KMs | 2 |
| 53 | Angamali (AFK) | 12:19 AM | 12:20 AM | 1 Mins | 448 KMs | 2 |
| 54 | Thrisur (TCR) | 01:23 AM | 01:25 AM | 2 Mins | 494 KMs | 2 |
| 55 | Guruvayur (GUV) | 02:10 AM | Destination | 0 Mins | 516 KMs | 2 |