| Train | Source | Destination | Days Run from Tambaram |
| 16101 - Ms Qln Express | Tambaram (TBM) | Kollam Jn (QLN) | Mon Tue Wed Fri Sat Sun |
| # | Station | Arrival | Departure | Halt | Distance | Day |
| 1 | Tambaram (TBM) | Source | 05:15 PM | 0 Mins | 0 KMs | 1 |
| 2 | Chengalpattu Jn. (CGL) | 05:43 PM | 05:45 PM | 2 Mins | 31 KMs | 1 |
| 3 | Villupuram Jn (VM) | 07:10 PM | 07:15 PM | 5 Mins | 134 KMs | 1 |
| 4 | Ulundurpet (ULU) | 07:42 PM | 07:43 PM | 1 Mins | 169 KMs | 1 |
| 5 | Vriddhachalam Jn (VRI) | 07:56 PM | 07:58 PM | 2 Mins | 188 KMs | 1 |
| 6 | Tiruchchirappalli Jn. (TPJ) | 09:40 PM | 09:45 PM | 5 Mins | 311 KMs | 1 |
| 7 | Dindigul Jn (DG) | 10:47 PM | 10:50 PM | 3 Mins | 405 KMs | 1 |
| 8 | Madurai Jn (MDU) | 12:10 AM | 12:15 AM | 5 Mins | 471 KMs | 2 |
| 9 | Virudunagar Jn (VPT) | 12:48 AM | 12:50 AM | 2 Mins | 515 KMs | 2 |
| 10 | Sivakasi (SVKS) | 01:13 AM | 01:15 AM | 2 Mins | 539 KMs | 2 |
| 11 | Srivilliputtur (SVPR) | 01:28 AM | 01:30 AM | 2 Mins | 556 KMs | 2 |
| 12 | Rajapalayam (RJPM) | 01:43 AM | 01:45 AM | 2 Mins | 567 KMs | 2 |
| 13 | Sankarankovil (SNKL) | 02:08 AM | 02:10 AM | 2 Mins | 600 KMs | 2 |
| 14 | Kadayanallur (KDNL) | 02:28 AM | 02:30 AM | 2 Mins | 621 KMs | 2 |
| 15 | Tenkasi Jn (TSI) | 02:48 AM | 02:50 AM | 2 Mins | 637 KMs | 2 |
| 16 | Sengottai (SCT) | 03:25 AM | 03:30 AM | 5 Mins | 645 KMs | 2 |
| 17 | Aryankavu (AYV) | 03:54 AM | 03:55 AM | 1 Mins | 660 KMs | 2 |
| 18 | Tenmalai (TML) | 04:23 AM | 04:25 AM | 2 Mins | 672 KMs | 2 |
| 19 | Punalur (PUU) | 05:25 AM | 05:30 AM | 5 Mins | 694 KMs | 2 |
| 20 | Auvaneswsarem (AVS) | 05:39 AM | 05:40 AM | 1 Mins | 702 KMs | 2 |
| 21 | Kottarakara (KKZ) | 05:49 AM | 05:50 AM | 1 Mins | 714 KMs | 2 |
| 22 | Kundara (KUV) | 05:59 AM | 06:00 AM | 1 Mins | 725 KMs | 2 |
| 23 | Kollam Jn (QLN) | 07:00 AM | Destination | 0 Mins | 738 KMs | 2 |